soluble cd14 scd14  (Elabscience Biotechnology)

Journal: Journal of Diabetes

Article Title: Inflammation in Diabetic Kidney Disease Is Linked to Gut Dysbiosis and Metabolite Imbalance

doi: 10.1111/1753-0407.70175

Figure Lengend Snippet: Systemic inflammation was associated with impaired intestinal barrier function in DKD patients. (A, B) Plasma TNF‐α and IL‐6 were analyzed by ELISA measurements. (C, D) Gut barrier function was assessed using ZO‐1 and sCD14 ELISA measurements in plasma. (E–H) Inflammation and leaky gut indicators were associated using Spearman correlation analysis. * p ≤ 0.05; ** p ≤ 0.01.

Article Snippet: Tumor necrosis factor‐a (TNF‐α), interleukin‐6 (IL‐6), zona occludens‐1 (ZO‐1), and soluble CD14 (sCD14) were analyzed in patients' serum using the human TNF‐α, IL‐6, ZO‐1, and sCD14 ELISA kit (Elabscience, China), according to the manufacturer's protocol.

Techniques: Clinical Proteomics, Enzyme-linked Immunosorbent Assay

Journal: Frontiers in Immunology

Article Title: Persistent innate immune dysfunction and ZIKV replication in the gastrointestinal tract during SIV infection in pigtail macaques

doi: 10.3389/fimmu.2025.1535807

Figure Lengend Snippet: PBMC from SIV-infected PTM are less permissive to in vitro ZIKV infection. (A, B) Peripheral blood mononuclear cells (PBMC) were isolated from pigtail macaques prior to and at 2 and 6 weeks post-SIV infection and infected in vitro with ZIKV Brazil at a multiplicity of infection (MOI) of 2. Cells and supernatant were harvested 4, 24, and 48 hours post-infection. (A) Quantitative real-time PCR (qRT-PCR) for ZIKV RNA in PBMC. (B) Plaque assay for infectious Zika virus. (A, B) Medians with interquartile ranges are shown. Kruskal-Wallis test versus pre-SIV levels, p-values * ≤ 0.05. (C) Frequency of CD16+CD14+ monocytes and macrophages (Mon&Mac) (top panel) and dendritic cells (lower panel) in blood from uninfected and SIV-infected pigtail macaques. Wilcoxon matched-pairs signed rank test, p-values ≤ 0.05 considered significant. (D) Gene expression of PBMC in blood at Week 2 post-SIV (top panel) and Week 6 post-SIV (bottom panel). t-test between each timepoint relative to pre-SIV, p-values *<0.01 shown by orange dots.

Article Snippet: Plasma and/or CSF quantification by ELISA of human soluble CD14 (sCD14), human fatty acid binding protein 2 (FABP2) (Fisher Scientific, Waltham, MA) or human LPS binding protein (LBP) (Biometec, Germany) was performed per the manufacturer’s instruction.

Techniques: Infection, In Vitro, Isolation, Real-time Polymerase Chain Reaction, Quantitative RT-PCR, Plaque Assay, Virus, Gene Expression

Journal: Frontiers in Immunology

Article Title: Persistent innate immune dysfunction and ZIKV replication in the gastrointestinal tract during SIV infection in pigtail macaques

doi: 10.3389/fimmu.2025.1535807

Figure Lengend Snippet: Post-ZIKV recruitment of CD16+ monocytes and macrophages is dampened in the periphery, but enhanced in tissues in SIV-infected macaques. (A) Frequency of CD16+CD14+ monocytes and macrophages (Mon&Mac) in blood (left panel), rectum (center panel), and peripheral lymph node (right panel) after ZIKV infection. (B) Frequency of neutrophils in blood after ZIKV infection. (C) Concentration of myeloperoxidase (MPO) in plasma as measured by ELISA. (A-C) Medians with interquartile ranges are shown. Mann-Whitney test between groups, p-values * ≤ 0.05.

Article Snippet: Plasma and/or CSF quantification by ELISA of human soluble CD14 (sCD14), human fatty acid binding protein 2 (FABP2) (Fisher Scientific, Waltham, MA) or human LPS binding protein (LBP) (Biometec, Germany) was performed per the manufacturer’s instruction.

Techniques: Infection, Concentration Assay, Clinical Proteomics, Enzyme-linked Immunosorbent Assay, MANN-WHITNEY

Journal: Viruses

Article Title: Elevated Inflammation Associated with Markers of Neutrophil Function and Gastrointestinal Disruption in Pilot Study of Plasmodium fragile Co-Infection of ART-Treated SIVmac239+ Rhesus Macaques

doi: 10.3390/v16071036

Figure Lengend Snippet: Minimal disruption of peripheral neutrophil frequencies and percentages of neutrophils undergoing apoptosis during P. fragile co-infection of ART-treated SIVmac239-infected rhesus macaques. Total neutrophil frequencies and frequencies of neutrophils undergoing apoptosis were assessed in whole blood before and after P. fragile co-infection of ART-treated SIVmac239-infected rhesus macaques (RMs) (n = 4) by flow cytometry. ( A ) Neutrophil (HLA-DR-CD11b+CD66abce+CD14+) frequency of live CD45+ cells was assessed throughout co-infection. ( B ) The frequency of neutrophils undergoing apoptosis (caspase3+) was assessed throughout co-infection. In both panels, each RM is represented by a different symbol and color. Baseline (BL) is the average of data collected at weeks 6, 4, 2, and 0 post-SIV infection (p.i.). Inoculation with SIVmac239 at week 0 p.i. is indicated by a purple dashed arrow. Inoculation with P. fragile at week 12 p.i. is indicated by a blue dashed arrow. Antiretroviral therapy (ART) was initiated at week 8 p.i., indicated by the dark-grey bar. Antimalarial treatment occurred throughout week 14 p.i., indicated by the light-grey bar. Statistical significance between all timepoints was calculated using a mixed-effects analysis with the Geisser–Greenhouse correction and a Tukey’s multiple-comparison test, with individual variances computed for each comparison. Significant multiplicity-adjusted p values are shown above horizontal black bars.

Article Snippet: Commercially available enzyme-linked immunosorbent assay (ELISA) kits were used to quantify the plasma levels of neutrophil granule components, including myeloperoxidase (MPO) (Abcam, Cambridge, UK); cathepsin G (MyBiosource, San Diego, CA, USA); proteinase 3 (PR3) (MyBiosource); biomarkers of NET formation, including citrullinated histone 3 (CitH3) (Cayman Chemicals, Ann Arbor, MI, USA) and neutrophil elastase (NE) (LSBio, Lynwood, WA, USA); markers of GI barrier permeability, including Zonulin-1 (MyBiosource) and intestinal fatty acid-binding protein (IFAB-P) (Novus Biologicals, Centennial, CO, USA); and surrogate markers of microbial translocation, including lipopolysaccharide (LPS)-binding protein (LBP) (Novus Biologicals) and soluble CD14 (sCD14) ELISA (ThermoFisher), as per the manufacturers’ recommended protocols.

Techniques: Disruption, Infection, Flow Cytometry, Comparison

Journal: Viruses

Article Title: Elevated Inflammation Associated with Markers of Neutrophil Function and Gastrointestinal Disruption in Pilot Study of Plasmodium fragile Co-Infection of ART-Treated SIVmac239+ Rhesus Macaques

doi: 10.3390/v16071036

Figure Lengend Snippet: Increased levels of microbial translocation and gastrointestinal (GI) barrier permeability markers during P. fragile co-infection of ART-treated SIVmac239-infected rhesus macaques. Markers of microbial translocation and GI barrier permeability were measured throughout P. fragile co-infection of ART-treated SIVmac239-infected rhesus macaques (RMs) (n = 4) via ELISA. Zonulin ( A ), intestinal fatty acid-binding protein (I-FABP; B ), Soluble CD14 (sCD14; C ), and LPS-binding protein (LBP; D ) levels were measured in plasma by ELISA. In all panels, each RM is represented by a different symbol and color. Baseline (BL) is the average of data collected at weeks 6, 2, and 0 post-SIV infection (p.i.). Inoculation with SIVmac239 at week 0 p.i. is indicated by a purple dashed arrow. Inoculation with P. fragile at week 12 p.i. is indicated by a blue dashed arrow. Antiretroviral therapy (ART) was initiated at week 8 p.i., indicated by the dark-grey bar. Antimalarial treatment occurred throughout week 14 p.i., indicated by the light-grey bar. Statistical significance between all timepoints was calculated using a mixed-effects analysis with the Geisser–Greenhouse correction and a Tukey’s multiple-comparison test, with individual variances computed for each comparison. Significant multiplicity-adjusted p values are shown above horizontal black bars.

Article Snippet: Commercially available enzyme-linked immunosorbent assay (ELISA) kits were used to quantify the plasma levels of neutrophil granule components, including myeloperoxidase (MPO) (Abcam, Cambridge, UK); cathepsin G (MyBiosource, San Diego, CA, USA); proteinase 3 (PR3) (MyBiosource); biomarkers of NET formation, including citrullinated histone 3 (CitH3) (Cayman Chemicals, Ann Arbor, MI, USA) and neutrophil elastase (NE) (LSBio, Lynwood, WA, USA); markers of GI barrier permeability, including Zonulin-1 (MyBiosource) and intestinal fatty acid-binding protein (IFAB-P) (Novus Biologicals, Centennial, CO, USA); and surrogate markers of microbial translocation, including lipopolysaccharide (LPS)-binding protein (LBP) (Novus Biologicals) and soluble CD14 (sCD14) ELISA (ThermoFisher), as per the manufacturers’ recommended protocols.

Techniques: Translocation Assay, Permeability, Infection, Enzyme-linked Immunosorbent Assay, Binding Assay, Comparison